Introduction. The irrational use of antibiotics is a determining factor for the development of antimicrobial resistance and the occurrence of healthcare-associated infections. In recent years, strategies have been adopted to improve the control of bacterial infections and the monitoring of antibiotic consumption at national level [1, 2]. The aim of the study was the retrospective analysis of the range of antibiotics prescribed to patients admitted to a hospital in Bucharest, between January-December 2019 and April 2022-March 2023. The consumption of antibiotics was expressed as DDD (defined daily dose)/ 1000 bed-days. Results and conclusions. An increasing trend was observed comparing the two studied time intervals, with high differences for the following therapeutic classes: combinations of penicillins with beta-lactamase inhibitors (from 4.64 to 38.99 DDD/1000 bed-days); cephalosporins (from 215 to 334.80 DDD/1000 bed-days); quinolones (from 15.18 to 48.54 DDD/1000 bed-days); carbapenems (from 20.14 to 58.47 DDD/1000 bed-days). Antibiotic consumption decreased for penicillins (from 4.64 to 3.66 DDD/1000 bed-days) and lincomycin (from 0.29 to 0.13 DDD/1000 bed-days). An increase in antibiotic consumption was observed for most antibiotic classes at hospital level. The continuous monitoring of prescription patterns provides important information to policy makers for the implementation of measures to reduce the irrational consumption of antibiotics, with the risk of the emergence of resistant strains.