A studywas carried out in the central and southern zones of wine growing of the Republic of Moldova during the period of 2007-2009 in order to evaluate the influence of biological active substances on the productivity of vines and quality of grapes. It was established that the treatment of inflorescence of the seed less Loose Perlette, Summer Muscat, Monukka,Thomps on Seed less, Ruby Seed less and seed Cardinal, Black Magic (Codreanca), Muscat Hamburg, Italia table grape varieties with biological active substances - gibberellic acid(GA3) - leads to an increase in size and weight of clusters and berries, productivity of vines and grapes quality.Productivity of vines increases by 10,1-92,3% and depends on biological peculiarities of grape varieties, concentration of growth regulators and terms of their application. We have established that for seedless grape varieties the optimal concentration of biological active substances in phases of post fertilization (3-5 days after flowering) is GA3-100 ppm, and for seed grape varieties-GA3-50ppm.