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 1059 42 |
| Ultima descărcare din IBN: 2023-03-26 14:57 |
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| 575.22:581.5 (1) |
| Genetică generală. Citogenetică generală (429) |
| Habiturile plantelor. Comportamentul plantelor. Ecologia plantelor. Etologia plantelor. Plantele şi mediul lor. (178) |
 SM ISO690:2012PIRKO, N., DEMKOVYCH, A., KALAFAT, L., PRIVALIKHIN, S., RABOKON, A., PIRKO, Ya., BLUME, Ya.. Intron length polymorphism of β-tubulin genes in different representatives of Pinaceae Lindl. family. In: Revista Botanică, 2016, nr. 2(13), pp. 5-9. ISSN 1857-2367. |
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| Revista Botanică | ||||||
| Numărul 2(13) / 2016 / ISSN 1857-2367 /ISSNe 2587-3814 | ||||||
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| CZU: 575.22:581.5 | ||||||
| Pag. 5-9 | ||||||
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Intron lengths polymorphism of β-tubulin genes was studied using TBP-method in four species of conifers of Pinaceae L. family (Pinus sylvestris L., Abies alba Mill., Picea abies (L.) H. Karst., Picea pungens Engelm.). Unique patterns were identified and molecular profiles for each of these species were created. It was established that the main distribution area of amplicons ranged from 301 bp to 1133 bp. There were 57 amplified fragments of different lengths. The greatest amount of amplicons (25) was found in Abies alba. Also this species had a rather high РІС value (0.354) compared to other studied representatives of Pinaceae family. The lowest values of РІС were observed in P. рungens (0.063) and P. abies (0.107). Analysis of Pinus sylvestris by ТВР-markers has shown that 14 fragments out of 18 analyzed ones were polymorphic and only 4 were monomorphic. Evaluation of РІС polymorphism in this species was 0.313. Shannon information index and expected heterozygosity, calculated using ТВР analysis, were highest in Abies alba (0.472 and 0.311) and Pinus sylvestris (0.400 and 0.265), respectively, and the smallest in Picea pungens (0.083 and 0.056). |
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