Preserving organism biodiversity is possible by genepool cryoconservation, which is followed by fundamental researches to elucidate the mechanism of cryodestruction and cryoprotection in different level of organization of biological objects, including in the spermatozoa’s membrane structures. First, at lipids level, which are essential and integral components of membrane and perform important functions in the viability of biological objects – energy source, biological active substance. Our reserches focused on determining of lipids in rooster and boar spermatozoa membrane in cryoconservation process. The results have elucidated lipid spectrum in rooster and boar spermatozoa plasma membrane in cryoconsevation process. Experimentally was established a significant decrease of the cholesterol:phospholipids proportion value in the freezing-defrosting process of the rooster sperm. It was ascertained that increase of the cholesterol:phospholipids proportion in the cryoconservation process of boar sperm is predeterminated by the cholesterol and phospholipids interactions of the synthetic mediums and structurals elements of the plasma membrane.