Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
286 3 |
Ultima descărcare din IBN: 2022-12-02 13:50 |
Căutarea după subiecte similare conform CZU |
616.72-018.3-089.844-092.9 (2) |
Patologia organelor de locomoţie. Sistemul osos şi locomotor (472) |
SM ISO690:2012 COBZAC, Vitalie, VERESTIUC, Liliana, JIAN, Mariana, NACU, Viorel. Chondrocytes isolation from hyaline cartilage by continuous monitoring method. In: Moldovan Medical Journal, 2021, nr. 6(64), pp. 13-19. ISSN 2537-6373. DOI: https://doi.org/10.52418/moldovan-med-j.64-6.21.03 |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Moldovan Medical Journal | ||||||
Numărul 6(64) / 2021 / ISSN 2537-6373 /ISSNe 2537-6381 | ||||||
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DOI:https://doi.org/10.52418/moldovan-med-j.64-6.21.03 | ||||||
CZU: 616.72-018.3-089.844-092.9 | ||||||
Pag. 13-19 | ||||||
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Background: Articular cartilage has poor regenerative capacities. Numerous cartilage repair techniques are known, including implantation of autologous chondrocytes. Material and methods: From 18 rabbits pieces of cartilage were harvested from femoral condyle. Minced cartilage was treated with 0.25% trypsin-EDTA. In the 1st group (n=9) the cartilage was digested with 0.6% collagenase in 15 ml tubes by shaking in incubator at 37°C, 5%CO2. In the 2nd group (n=9) digestion was performed in 25cm2 cell culture flasks placed on the lateral side, monitoring the process under a microscope after 120 minutes. The isolated cells were cultured to a 80-90% confluence. The chondrocytes were identified using histochemical staining after culturing for 16 days in overconfluence. Results: Chondrocytes isolation in the 1st group lasted a fixed 360 minutes, in the 2nd group – 140±10 minutes. In the 1st group were isolated 9.2x104±3.1x104 chondrocytes with a viability of 85.36±16.41%, but in the 2nd group – 1.6x105±3.4x104 chondrocytes with a viability of 98.09±3.85%. The mean period of cell culture in the 1st group was 15±2 days, in the 2nd group – 11±3 days. In first passage of the 1st group were obtained – 1.2x106 ±4.3x105 chondrocytes and in the 2nd group – 2.92x106 ±3.6x105 chondrocytes. The secreted extracellular matrix by chondrocytes was stained specifically for cartilaginous tissue. Conclusions: The method used for chondrocytes isolation has a direct impact on the number of isolated cells, their viability, but also upon the culture period and the number of cells obtained during the first passage. |
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Cuvinte-cheie cartilage, chondrocytes isolation, continuous monitoring |
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